Shondra Miller, PhD

Shondra Miller, PhD
Assistant Member, Department of Cell and Molecular Biology
Founding Director, Center for Advanced Genome Engineering
St. Jude Children’s Research Hospital

Shondra Miller, PhD is an Assistant Member in the Department of Cell and Molecular Biology and the Founding Director of the Center for Advanced Genome Engineering at St. Jude Children’s Research Hospital in Memphis, TN.
Shondra completed her PhD in Cell and Molecular Biology from The University of Texas Southwestern Medical Center in August 2008. While at UT Southwestern, she worked in Matthew Porteus’ lab on the optimization of zinc finger nucleases for use in mammalian cells. After graduate school, she was recruited to Sigma-Aldrich as a senior scientist in R&D working on their Compozr ZFN technology. In 2012, she returned back to academia as the Founder and Director of the Genome Engineering and iPSC Center (GEiC) at Washington University School of Medicine in St. Louis, where she and her team of 13 scientists completed thousands of genome editing projects using CRISPR-Cas9. Just last year, she joined the Faculty at St. Jude Children’s Research Hospital where she set up and is directing the Center for Advanced Genome Engineering (CAGE).

High-Efficiency Genome Editing Guided by Targeted Deep Sequencing

Genome Engineering allows the easy manipulation of genomes down to the nucleotide level.  Targeted deep sequencing enables the detection and quantification of low-frequency editing events.  However, the large amounts of data generated by targeted deep sequencing can be difficult to interpret and quickly analyze.  We have developed a Python-based computer program called CRIS.py that allows the easy analysis of multiple types of editing events.  We will show examples of how rapid deep sequence analysis has guided experimental design leading to high-efficiency genome editing in a broad range of applications.